Biology and Radiobiology of Anucleate Systems. Plant Cells by Silvano Bonotto

By Silvano Bonotto

Biology and Radiobiology of Anucleate platforms: II. Plant Cells encompasses the lawsuits of foreign Symposium held in Mol, June 21-23, 1971. The symposium is prepared via the dept of Radiobiology, Centre d'Etude de I'Energie Nucleaire (C.E.N./S.C.K.), Mol (Belgium) less than the auspices of the fee of the ecu groups (EURATOM) and of the ""Relations Culturelles Internationales"" (Brussels). the purpose of the three-day symposium is to compile, for the 1st time, scientists engaged on anucleate structures received from micro organism and animal or plant cells.
This quantity consists of sixteen papers pertaining to in particular to plant cells. each one paper is equipped in response to the date and consultation after they were provided.
This publication will offer helpful info for evaluating the houses of anucleate Acetabularia with these of different anucleate cells, which might be very precious for larger realizing of many difficulties in regards to the nucleocytoplasmic relationships and mobile differentiation in common and irradiated cells.

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Physiol. 41, 764-70. 51. M. (1970) - PI. , Lancaster 46, 13641. 52. F. I. (1968), Proc. natn. Acad. Sei. 59, 606-612. 53. Spencer,D. (1965) -Arch. Biochem. Biophys. Ill, 381390. 54. A. T. (1970) - Planta 94, 60-72. 55. R. T. (1970) - Planta 95, 227-46. 56. , Bunning,J. H. (1967) - PI. , Lancaster 42, 233-37. 57. A. R. (1971) - PI. , Lancaster 47, 189-91. 58. L. M. (1970) - PI. , Lancaster 45, 624-30. 59. Tsukamoto,A. (1970) - PI. Cell Physiol. 11, 221-30. 60. K. E. (1970) - J. Biol. Chem. 245, 5137-44.

The results reported in table 5 are the means of one day. The results of these experiments show that the ATP content, in the chloroplasts, decreases in the anucleate fragments as compared with whole algae. Comparison of the ATP content of the chloroplasts of Acetabularia anucleate for two different periods of time shows that there is no interpretable difference whether the algae have been recently anucleated or not (10 - 16 days as compared with 16 - 22 days). This suggests that the chloro- 60 ANUCLEATE SYSTEMS: PLANT CELLS plasts of the anucleate algae use more ATP than those of whole algae, but that a new steady-state is rapidly achieved.

The suspended drop culture was made by using a small volume of plastids removed from the culture vessel 24 hours after isolation. The first indication that division is about to occur is given by a dumb-bell shaped appearance of the plastid which remains in this configuration for several hours. The final separation into two daughter plastids takes place fairly rapidly, generally within one hour. During this time the constriction becomes tube-like with a diameter about one fifth that of the chloroplast.

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