Analysis of Triglycerides by Carter Litchfield

By Carter Litchfield

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3 3 3 3 3 3 3 3 2. Solvent Two general types of solvent mixtures have been widely used for separating triglycerides on AgN0 -impregnated TLC plates: (i) chloroform containing 0-6% methanol, ethanol, or acetic acid; and (ii) various benzene/diethyl ether mixtures. Typical solvent systems for separating triglyceride mixtures of varying unsaturation are listed in Table 4-1, and many related combinations can be found in the literature. The exact solvent mixture used for a specific separation varies slightly from one laboratory to another since R values for specific triglycerides are influenced by temperature, activity of the silicic acid, relative humidity, and AgNO content of the adsorbent (205).

SILVER ION ADSORPTION CHROMATOGRAPHY I. METHODS A. Choice of Method Both thin-layer and column chromatography can be used for the separation of triglycerides with a AgN0 3-impregnated adsorbent. The choice of method for fractionating a given sample depends on the type of resolution desired and the amount of material to be separated. Column operation has the advantage of a slightly larger sample size (80-150 mg) with less possibility for atmospheric oxidation; but columns are slower, more difficult to monitor, and generally give poorer resolution than TLC plates.

A d d 15 m l of K I solution and then sufficient NaoSoOa solution to discharge the blue color f r o m starch indicator. T h e b r o m i n a t e d triglycerides are isolated by adding 5 0 m l o f water and extracti n g with c h l o r o f o r m (480). * T h e greater solubility of brominated triglycerides in C H C 1 3 m a k e s it a better solvent than p e t r o l e u m ether for achieving c o m p l e t e b r o m i n a t i o n . REACTIONS AT DOUBLE I. BONDS 45 Conjugated double bonds and acetylenic bonds are not fully brominated by the procedure on page 44.

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